|New||Ant Trooper, Big Ant Trooper, Big Galoomba, Blockstepper, Blurker, Brolder, Cat Banzai Bill, Cat Bullet Bill, Cat Goomba, Charvaargh, Conkdor, Flopter, Fuzzler, Grumblump, Hop-Chop, Horned Ant Trooper, Ka-Thunk, Madpole, Parabones, Piranha Creeper, Rammerhead, Ring Burner, Skipsqueak, Snow Pokey, Spiny Skipsqueak, Splorch, Splounder, Ty-Foo, Walleye|
|Returning||Baddie Boxes, Banzai Bill, Banzai Bill Blaster, Beach Koopa, Biddybud, Big Boo, Bill Blast, Blooper, Blue Lava Bubble, Bob-omb, Boo, Boomerang Bro, Bullet Bill, Bully, Cannon, Cannonball, Chargin’ Chuck, Cheep-Cheep, Coin Coffer, Firebar, Fire Bro, Fire Piranha Plant, Fizzlit, Fuzzy, Galoomba, Goomba, Goomba Tower, Hammer Bro, Koopa Troopa, Lava Bubble, Magikoopa, Micro-Goomba, Octoomba, Para-Biddybud, Peepa, Piranha Plant, Big Piranha Plant, Porcupuffer, Raining Debris, Spike, Spiny, Stingby, Thwomp|
|Boss||Bowser, Boom Boom, Pom Pom, Hisstocrat, Boss Brolder, King Ka-Thunk, Prince Bully, Motley Bossblob|
Most enemies can be defeated by jumping on them once or multiple times, but others are invulnerable to this type of attack. Use the Cat or Tanooki Power-Up to swipe the enemy or the Fire Power-Up to shoot them. The Boomerang Power-Up is also useful and can clear multiple enemies at once. Obviously, the Super Star Power-Up will defeat all enemies simply by touching them but this is a limited Power-Up and cannot be easily obtained like the other Power-Ups.