Nate and Victor walk into a pub in London to meet a man named Talbot, who's interested in purchasing Nate's ring that belonged to Sir Frances Drake.
20 years earlier, a young Nathan seeks out the same ring in a museum in Cartagena, Columbia. Unbeknownst to him a woman by the name or Marlowe and her companion, Victor Sullivan, also seek the ring and catch Nathan. Luckily, Sully sees greatness in Nate and rescues him, taking him as a protege from that day forward.
Back in the present day, the group follow Talbot and Marlowe to an underground library and retrieve T. E. Lawrence's notebook that confirms Sir Frances Drake's secret voyage to Arabia. Commissioned by Queen Elizabeth I and John Dee, it is believed that Sir Frances' voyage was in search of the lost city of Ubar.
The group splits up - with Nate and Sully heading to a chateau in France while Chloe and Cutter make their way to a citadel in Syria, both of which contain a piece of a mysterious amulet that points the way to Yemen. In Yemen, Nate reluctantly seeks the aid of his estranged wife, Elena Fisher, to find the underground tomb and discover the location of the lost city within the vast Rub' al Khali desert.
After making his way to the Rub' al Khali desert, Nate gains help from a Bedouin named Salim. Salim warns Nate that the city of Ubar was doomed thousands of years ago by King Solomon when he imprisoned evil Djinn within a brass vessel and cast it into the heart of the city, but Nate continues forward in an attempt to rescue Sully.
Finding his way to the city of Ubar, Nate learns that the results of King Solomon's actions left the water tainted with minerals that cause vivid hallucinations agents. These agents are what Marlowe seeks to gain for her own evil purpose and the reason Sir Francis Drake abandoned his his quest long ago.
In an effort to stop Marlow, Nate accidentally blows up the central cistern of Iram and causes the city to begin to collapse around them. As Nate and Sully escape, they are corned by Talbot and Marlowe, but the floor gives way and Marlowe falls into a sinkhole along with Drake's ring. Finishing off Talbot as Ubar collapses, the pair escape and are rescued by Salim once again.