There are nine total Animal Sanctuaries in the Iki Island Expansion for Ghost of Tsushima: Director’s Cut. These are completely optional but if you’re aiming for all Trophies, must be completed towards that goal. These are a new type of collectable where Jin will play his flute for the specific animal that will result in a Charm that you can power up.
Deer Sanctuary #1 - Sly Hunter’s Forest
This Deer Sanctuary is found right in the center of the Sly Hunter’s Forest and is most likely the first you’ll encounter on Iki Island. You’ll spot a campfire with a group of Mongols you must take out first before you can access it.
Deer Sanctuary #2 - Shattered Cliffs
This Deer Sanctuary can be found by using the Fast Travel to Fune’s Refuge and using the exit shortcut and the entrance. From the position it takes you, follow it south and then drop down off the rocks to locate it.
Deer Sanctuary #3 - Zasho River Crossing
The final Deer Sanctuary requires you to complete the Zasho River Crossing Mongol Territory. Once complete, you’ll find the Deer Sanctuary in the center.
Cat Sanctuary #1 - East of Thunderhead Cliffs
The first Cat Sanctuary can be found by heading to Thunderhead Cliffs by way of the Memory of Sorrow Fast Travel and climbing down the cliff to the east. From here, head across the bridge and follow the path east and you’ll reach a ravine with some Mongols at the Sanctuary site.
Cat Sanctuary #2 - Southeast of Katayama Market
The next Cat Sanctuary can be found by heading toward the river to the southeast of Katayama Market. One of the Main Tales will take you right next to this Sanctuary making it very difficult to miss. Once you sit down to play the flute, you’ll need to deal with an ambush of two Mongol Shamans and three dogs.
Cat Sanctuary #3 - East of Old Gambler’s Woods
The final Cat Sanctuary is close to the previous one, located to the west of that Sanctuary and the east side of Old Gambler’s Woods, close to the river. Once again, you’ll need to deal with a group of Mongols that occupy the Sanctuary before using your Flute.
Monkey Sanctuary #1 - Island east of Raider’s Promontory
The first Monkey Sanctuary can be found on the island east of Raider’s Promontory, located off the coast to the north of Sly Hunter’s Forest. You’ll need to dispatch a group of Mongols at the caged Monkey’s before you can use your flute.
Monkey Sanctuary #2 - Southwest of Smuggler’s Wood
The next Monkey Sanctuary is located on the south of Iki Island, in the southwest of Smuggler’s Wood, a short distance west from the Saruiwa Overlook Mongol Territory.
Monkey Sanctuary #3 - Saruiwa Island
The final Monkey Sanctuary is located to on the southwest island of Saruiwa. You’ll have to reach it via Sarubashi to the north, accessed by heading southwest on Gonoura Cape. You’ll need to scale the mountain to the top to reach the Sanctuary, look for two Monkey statues on the north of the island to start the climb. Once you claim your final Sanctuary, you’ll also unlock Chiyoko's Song.